Development of Instantaneous Color Holography System for Sensing Fluorescence and White Light
The National Institute of Information and Communications Technology (NICT), the Japan Science and Technology Agency (JST), Toin University of Yokohama, and Chiba University have succeeded in developing a color-multiplexed holography system by which 3D information of objects illuminated by a white-light lamp and self-luminous specimens are recorded as a single multicolor hologram by a specially designed and developed monochrome image sensor. Single-shot color-multiplexed fluorescence holographic microscopy is realized by exploiting digital holography and computational coherent superposition (CCS), with the latter having been proposed by NICT. The developed microscope acquires the color 3D information of self-luminous objects with a single-shot exposure and no color filter array. The developed system does not need a laser light source and has the capability to conduct multicolor 3D motion-picture sensing of biological samples and moving objects at video rate. Further development will be conducted toward multicolor 3D microscopy of extremely weak light such as autofluorescence light and nonlinear light.
This achievement was published in Applied Physics Letters as an open-access paper on July 22, 2020.
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